关闭

关闭

关闭

封号提示

内容

北京pk10是国家彩票吗 【doc】中国红豆杉紫杉烷10β-羟化酶的分子克隆及在酿酒酵母中的表达

北京赛车微信实力群:【doc】中国红豆杉紫杉烷10β-羟化酶的分子克隆及在酿酒酵母中的表达.doc

【doc】中国红豆杉紫杉烷10β-羟化酶的分子克隆及在酿酒酵母…

jiao金玲
2018-11-08 0人阅读 0 0 0 暂无简介 举报

北京pk10是国家彩票吗 www.qdpo.net 简介:本文档为《【doc】中国红豆杉紫杉烷10β-羟化酶的分子克隆及在酿酒酵母中的表达doc》,可适用于项目管理领域

【doc】中国红豆杉紫杉烷β羟化酶的分子克隆及在酿酒酵母中的表达中红豆杉紫杉红国红化红的分子克隆及β在红酒酵母中的表达第卷第期年月红中红红大学JournalofHuazhongAgricuhuralUniversityVoINoFeb~TaxolBiosynthesis:MolecularCloningofaCytochromePTaxane~hydroxylasecDNAfromTaxuschinensisand,ExpressioninSaccharomycescerevisiaeTuJunZhuPingChengKedi(KeylaboratoryofBiosynthesisofNaturalProducts,MinistryofPublicHealthInstituteofMateHaMedicaChineseAcademyofVIedicalSciencesPekingUnionMedicalCollege,Beijing,China)ThenaturalproductTaxol,producedbyTaxusspecies,isanimportantanticancerdrugThelowyieldofTaxolfromnaturalresourcesandthelackofacommericallyviabletotalsynthesishaverenderedthatTaxolisproducedprimarilybysemisynthesismethodsThebiosynthesisofTaxolfromplantprima一rymetabolismisaverycomplexprocess,includingnearstepTaxolbiosyntheticpathwaystoconstructitstetracyclicskeletonandtheadditionofthevariousoxygenandacylfunctionalgroupsAcentralfeatureinthebiosynthesisofTaxolisoxygenationatmultiplepositionsofthetaxanecorestructure一reactionsthatareconsideredtobemediatedbycytochromePdependentmonooxygenasesSeveral一fulllengthcytochromePdependentmonooxygenasesequenceswereobtainedfromacDNAlibraryconstructedfrommRNAisolatedfromTaXUSchinensiscallusculturecellsAllT”sspeciesareknowntobeverycloselyrelated,sowemaydesignedseveralpairsofprimerstoclonefulllengthtaxanephydroxylasegenefromchinensisaccordingtothebothendsoftaxane一hydroxylasesequencesfromcuspidataAdistinctivePCRproductwithexpectedsizeofkbwasamplifiedbvPCRscreeningchinensiscDNAlibraryThePCRproductwasclonedintorende一dvectorDMDT(TaKaRa)forDNAsequencingThisdeterminationhasbeenperformedonthestrandstoconfirmtheaccurracyofsequenceThefulllengthcDNAcontainsabpopenreadingframe,whichencodesaminoacidsdeducedproteinofmolecularweightDawiththeoreticalpofThededucedaminoacidsequencesincludesanN,terminalhydrophobichelixrequiredforanchoringinendoplasmicreticulum(ER)membrane,aprolichrichhingeregionmodulatingfoldingandsubstrateaccesstothecatalyticpocket,precededbyaclusterofbasicresidues(thehalttransfersigna)betweenthehydrophobicaminoterminalmembraneanchoringsegementandtheglobularpartoftheproteinThe,deducedaminoacidsequencecomprisesseveralofsignaturefeaturesofothercytochromePOdependentmonooxygenasesofplantoriginincludingThePF(GIRTCPGdomainofthisputativeproteincontainsthemostconsenSUSsequence(FxxRCXG)withtheabsolutelyconservedcysteinethatservesasfifthligandtohemeirontheESIRdomain,hastheabsolutelyconservedEXxRmotifinhelixK,andthePSRFdomainformanERRtriadthatisthoughttoformasaltbridgethatlockstheCvspocketinpositionandassureshemeassociationwiththeproteinandfinally,theASYDTTdomain,containsahighlyconservedThrthatisthoughttobeinvolvedinbindingandactivation,whichcorrespondstotheDrotonReceiveddate:*SupportedbyNational(GrantNumber)**CorrespondingauthorTuJun,fen~le,bornin,HighTechnologyPrograms(GrantNumberAA)andtheNationalScienceFbundationofChnaPhDstudmentinChineseAcademyofMedi~lSciencesandPekingUnionMedicalCollege,Beijing红中红红大第学卷transfergrooveonthedistalsideoftheheineComparisonwiththatofcytochromePtaxanehydroxylasecDNAfromTaxus(uspidatarevealedsignificanthomology(identity)andindicated,theyhaveanverycloserrelationshipl’heSac’(‘haromyc’cerevisiaeWHIemployedastheexpressionhost,whengrownongalactose,expressestheHTRgeneencodinganHelianthustuberosusNADPHcytochromePreductasewhichisessentialforfunctionalcatalysisbythecoexpressedcytochromePmonooxygenaseWhensuitablesubstratesfortestoffunctionareavailable,functionofexpressionofthistargetenzymesPclonewillfollowconfirmationCloningandexpressionofnewTaxolbiosynthetichydroxylasegenesnotonlyimprovedetailunderstandingofbiosyntheticpathwayofTaxolbutalsoimprovetheproductionyieldsandobtainnoveltaxanemetabolitesandderiativesbybioengineeringmanipulationsKeywordsTaxolbiosynthesiscytochromePmonooxygenasetaxanelOhydroxylaseTaxuschinensisSaccharomycescerevisiaeaCNQ中红豆杉紫杉红国红化红的分子克隆及在红酒酵母中的表达p涂琚棣朱平程克中科院国医学学中红和科大红物究所国医学研红生部天然红物生物合(,,成重点红红室北京,)天然红物紫杉醇是一红重要的抗癌红其天然源的红乏和缺少商红上可来行的化全合成促红了红学紫杉醇生物源的深入究来研紫杉醇的生物合成是一红红的多步红程个,主要包括四红骨架的建和加构入各红红基和红基基红其中红基的加人由红胞色素氧化红催化我红从,P中红豆杉愈红红红红国胞构建的文红中克隆得到几个片段其中一mRNAeDNAPeDNA个红的片段红bpeDNA,红个氨基酸推蛋白分子量红断等红点红序列比红红示红,I)a,,个断个推蛋白包含多红胞色素氧区化红的保守具有典型的红胞色素氧化红的特征红P,P,一步的比红红红其已红定与的红北红豆杉紫杉红一红化红有的同源性将个红红片段红接Of}eDNA在红粒上建表构pYeDP达体红红红入相红的酵母表菌株达和红行表达红得相红大小的,wHTWVS,蛋白表红达条功能红定的,工作正在红行中红红红紫杉醇生物合成红胞色素氧化红紫杉红一红化红中红豆杉国O红酒酵母任红红红红京(Or:)

用户评价(0)

关闭

新课改视野下建构高中语文教学实验成果报告(32KB)

抱歉,积分不足下载失败,请稍后再试!

提示

试读已结束,如需要继续阅读或者下载,敬请购买!

评分:

/6

意见
反馈

立即扫码关注

爱问共享资料微信公众号

北京pk10是国家彩票吗

举报
资料